To validate and calibrate the SUCCEED FBT, test results have been compared with visual observations of gastric and colonic tissue samples from cadavers of equine subjects following necropsy. To assess the FBT’s predictive value, Test A (albumin) was compared to colonic lesions grade 1 or higher, while Test H (hemoglobin) was compared to lesions grade 2 or higher (where whole blood was visible) in both the stomach and colon.
Test Detection Limits |
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| Test A (Albumin | Test H (Hemoglobin) | |
| Lower Detection Limit | 8 ppm | 8 ppm |
| Upper Detection Limit | 10,000+ ppm | 10,000+ ppm |
This study was completed in October, 2011. Results show that the FBT is a reliable test for both gastric and colonic injury, and is effective in distinguishing the two.
Analysis of Tests A and H as indicators of ulceration |
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| N=178 | Test A (colon) | Test H (full GI tract) |
| True Positives | 166 | 154 |
| True Negatives | 3 | 11 |
| False Positives | 8 | 5 |
| False Negatives | 1 | 8 |
| Sensitivity | 99.4% | 95.1% |
| Specificity | 27.3% | 68.8% |
| Positive Predictive Value | 95.4% | 96.9% |
| Negative Predictive Value | 75.0% | 57.9% |
| P-value | .045 | .028 |
