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If a solid line appears only at the CTL point (Control line) on either Test A or Test H, this is a negative result. Note that some color may appear along the edges of the TST point. This is still a negative test. If a complete, solid line appears at the TST point (Test line) on either or both Test A and Test H, that is a positive test result. (See illustrations at right.) Note — a positive test line for Albumin (Test A) will typically be lighter than the Hemoglobin test line or the Control lines.

The following chart summarizes the meaning of positive and/or negative test results across the SUCCEED® FBT tests A and H.

Figure 1 — Summary of Test Results

*Note that Test A uses an antibody for albumin, which typically breaks down in digestion, particularly when exposed to digestive enzymes in the proximal portion of the small intestine. Thus a positive Test A will indicate bleeding from a source caudal to the duodenum (e.g. hindgut). Also, because albumin may be present at the site of low-grade injury absent whole blood, you may get a positive Test A in response to a low-grade lesion in the hindgut, such as may occur with heavy parasitism or disseminated grade 1 colonic ulcers.

Test H uses an antibody for hemoglobin, which is more resistant to enzymatic activity. Thus a positive Test H, with negative Test A, will indicate foregut blood loss. You will not obtain a positive result from either test A or H when a horse has a foregut lesion equivalent to a grade 1 gastric ulcer, where whole blood loss does not occur.

For more details, refer to the complete SUCCEED® FBT instruction sheet.